Composite

Part:BBa_K190033:Design

Designed by: Michael Verhoeven   Group: iGEM09_Groningen   (2009-09-07)
Revision as of 18:58, 18 October 2009 by PaulSchavemaker (Talk | contribs) (→‎Design Notes)

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GVP with ArsR regulated promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 414
    Illegal BamHI site found at 4219
    Illegal XhoI site found at 3907
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 865
    Illegal NgoMIV site found at 1267
    Illegal AgeI site found at 4186
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2677
    Illegal SapI.rc site found at 3833


Design Notes

Melbourne GVP cluster was used containing the 400bp repeat in gvpL.

Source

E.coli and Bacillus megaterium

References