Composite

Part:BBa_K190035:Design

Designed by: Michael Verhoeven   Group: iGEM09_Groningen   (2009-09-07)
Revision as of 18:56, 18 October 2009 by PaulSchavemaker (Talk | contribs) (→‎Design Notes)

GVP with CueO regulated promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 385
    Illegal BamHI site found at 4190
    Illegal XhoI site found at 3878
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 836
    Illegal NgoMIV site found at 1238
    Illegal AgeI site found at 4157
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2648
    Illegal SapI.rc site found at 3804


Design Notes

The Melbourne 2007 BBa_I750016 GVP biobrick was cloned behind a copper inducible promoter.

Source

E.coli and Bacillus

References