Part:BBa_K2706008

cymA

Endogenous to Shewanella Onedensis, cymA can accept electrons in interior of cell membrane from MtrCAB complex

CUG-China 2023–Contribution

Microbial electron exchange with extracellular substrates,such as electrodes in BESs, is often called microbialextracellular electron transfer (EET). The most wel lcharacterized microbial EET pathway is the Mtr pathway of S. oneidensis MR-1. S. oneidensis MR-1 transfers electrons from the quinone/quinol pool in the cytoplasmic membrane to the bacterial surface through redox and structural proteins,including the inner membrane c-Cyt CymA, the periplasmicc-Cyts Fcc3 and STC, and the outer membrane porin-c-Cytcomplex MtrCAB. Eventually, electrons are transferred from the bacterial surface to extracellular acceptors directly via the outer membrane c-Cyts or via nanowires, or indirectly via self-secreted electron mediator flavins. Shewanella nanowires are the extensions of the outer membrane and periplasm in which the cell surface c-Cyts MtrC and OmcA are the key players for long-distance electron transfer.

Fig. Extracellular electron transfer (EET) pathways of Shewanella oneidensis MR-1 to electrodes.

[1] Yidan Hu, Wang Yinghui, Han Xi, et al. Biofilm Biology and Engineering of Geobacter and Shewanella spp. for Energy Applications[J]. Frontiers in Bioengineering and Biotechnology, 2021, 9.

[2] Liang Shi, Dong Hailiang, Reguera Gemma, et al. Extracellular electron transfer mechanisms between microorganisms and minerals[J]. Nature Reviews Microbiology, 2016, 14(10): 651-662.

This contribution was added by the CUG-China 2023.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 306
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]