Part:BBa_K190028:Design

GlpF

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 283
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part has been pcr-ed out of the genome of E.coli 356 in two steps. First the whole gene was pcred out the genome and in the second step an EcoR1 restiction site was removed.

Source

This part comes from genomic DNA of the Escherichia coli 536.

References

Some useful papers about GlpF:

• [http://2009.igem.org/Team:Groningen/Literature#Meng2004 Meng 2004] (As(III) and Sb(III) Uptake by GlpF and Efflux by ArsB in Escherichia coli)
• [http://2009.igem.org/Team:Groningen/Literature#Rosen2009 Rosen 2009] (Transport pathways for arsenic and selenium: A minireview)
• [http://2009.igem.org/Team:Groningen/Literature#Porquet,A,et al.2007 Porquet, A, et al.2007] (structural similarity between As(OH)3 and glycerol)
• [http://2009.igem.orgTeam:Groningen/Literature#Fu,DX,et al.2000 Fu, DX, et al.2000] (Structure of the GlpF channel)
• [http://2009.igem.orgTeam:Groningen/Literature#Heller,KB,etal.1980 Heller, KB, et al.1980] (Glycerol transport properties of GlpF)