Regulatory
pArsR

Part:BBa_K190015

Designed by: Michael Verhoeven   Group: iGEM09_Groningen   (2009-07-15)
Revision as of 18:37, 18 October 2009 by PaulSchavemaker (Talk | contribs)

Arsenic Promoter (ArsR regulated)


This set includes promoters that are sensitive to various metals. The promoters are typically regulated by a receptor protein that binds to the metal ion or complex.

Promoter sequence with recognition site for ArsR transcriptional regulator protein. ArsR binds to the promoter sequence in the absence of As and releases on binding of As, thereby activating transcription (1).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


1) C. Xu, W. Shi, and B.P. Rosen (1996) The Chromosomal arsR Gene of Escherichia coli Encodes a trans-acting Metalloregulatory Protein, The Journal of Biological Chemistry, Vol. 271, No. 5, Issue of February 2, pp. 2427–2432.

[edit]
Categories
//function/regulation/transcriptional
//promoter
//regulation/negative
Parameters
negative_regulatorsbinding of As (III) to ArsR promotes dissociation of ArsR from the promoter region.