Coding

Part:BBa_K190028:Experience

Designed by: Jolanda Witteveen   Group: iGEM09_Groningen   (2009-08-26)
Revision as of 18:34, 18 October 2009 by JolandaWitteveen (Talk | contribs) (→‎User Reviews)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K190028

This part has been used to import As(III) into the E.coli cells. It has been used in combination with metal accumulation proteins to make a accumulation device. The two devices are BBa_K190038, a combination of GlpF with the accumulatin protein [http://2009.igem.org/Team:Groningen/Project/Accumulation#Metals fMT] and BBa_K190037, a combination of GlpF with the fusion protein of MBP with [http://2009.igem.org/Team:Groningen/Project/Accumulation#Metals ArsR] (an accumulation protein).

User Reviews

BBa_K190028 iGEM_Groningen2009

We did not have a lot of trouble with doing a pcr of GlpF to get it out of the genome or to get the EcoR1 and Pst1 site out. We used it in testing the import of arsenic by doing a accumulation assay and analyse it with ICP-MS. Protocols can be found [http:2009.igem.org/Team:Groningen/Protocols here.]




UNIQ7c8bf86ad1008a6f-partinfo-00000003-QINU UNIQ7c8bf86ad1008a6f-partinfo-00000004-QINU