Composite

Part:BBa_K4724075

Designed by: Xinyao Li   Group: iGEM23_NJTech-China-A   (2023-10-07)
Revision as of 03:18, 8 October 2023 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


NusA-LSPETase

We synthesized the pET-22b(+)-LSPET-12 plasmid from the company and will construct the recombinant plasmid by attaching this fusion tag NusA in front of the LSPETase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1368
    Illegal XhoI site found at 2344
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1600
    Illegal AgeI site found at 1687
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None