Part:BBa_K4719028

phaC-phaA-phaB-pKARA_RT3 operon for in situ dyed bacterial cellulose - polyhydroxybutyrate composite
Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 4992
  Illegal SpeI site found at 37
  Illegal PstI site found at 824
  Illegal PstI site found at 1397
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 4992
  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal SpeI site found at 37
  Illegal PstI site found at 824
  Illegal PstI site found at 1397
  Illegal NotI site found at 200
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 4992
  Illegal BglII site found at 642
  Illegal BamHI site found at 3039
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 4992
  Illegal SpeI site found at 37
  Illegal PstI site found at 824
  Illegal PstI site found at 1397
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 4992
  Illegal SpeI site found at 37
  Illegal PstI site found at 824
  Illegal PstI site found at 1397
  Illegal NgoMIV site found at 253
  Illegal NgoMIV site found at 368
  Illegal NgoMIV site found at 602
  Illegal NgoMIV site found at 914
  Illegal NgoMIV site found at 1193
  Illegal NgoMIV site found at 1606
  Illegal NgoMIV site found at 1673
  Illegal AgeI site found at 341
  Illegal AgeI site found at 4455
  
• 1000
  COMPATIBLE WITH RFC[1000]

Possible application as colorful biodegradable plastic.

Growth burden

In order to work with E. coli for designing constructs and testing synthetic biology systems, the growth burden of said synthetic biology tools has to be measured. We performed growth burden evaluation by measuring OD600 for five hours of modified and unmodified E. coli DH5α. The composite of in situ dyed PHB did not inhibit the growth of E. coli as seen in Figure 1.

Figure 1: growth burden of CBD-ProThr box-AnCDA composite.

Sequence and Features BBa_K4719028 SequenceAndFeatures