Composite

Part:BBa_K4604035:Design

Designed by: Hannah Swientek   Group: iGEM23_Freiburg   (2023-10-07)
Revision as of 22:35, 7 October 2023 by Registry (Talk | contribs)

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piG_08 (ampProm_mazE)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 226
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 515


Design Notes

Choice of a constitutive promoter to ensure baseline expression of MazE (antitoxin)


Source

The Amp promoter was taken by iGEM ECUST 2017 (BBa_K2308010). Assembled via a PCR annealing technique and Gibson Assembly.

References