Composite
Part:BBa_K4719020
Designed by: Auguste Stankeviciute Group: iGEM23_Vilnius-Lithuania (2023-09-07)
Revision as of 22:32, 7 October 2023 by Augustestankeviciute (Talk | contribs)
CBD-ProThr box-ArCE4A chitin deacetylase and cellulose binding domain fusion protein
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 432
Illegal NgoMIV site found at 961
Illegal AgeI site found at 417 - 1000COMPATIBLE WITH RFC[1000]
We fused a cellulose binding domain connected to a linker to the N-terminus of deacetylase ArCE4A to ensure a higher degree of deacetylation. For protein purification 6x his-tag was added to the N-terminus of cellulose binding domain.
Growth burden
In order to work with E. coli for designing constructs and testing synthetic biology systems, the growth burden of said synthetic biology tools has to be measured. We performed growth burden evaluation by measuring OD600 for five hours of modified and unmodified E. coli DH5α. The composite of recombinant deacetylase CBD-ProThr box-ArCE4A did not inhibit the growth of E. coli as seen in Figure 1.
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