Coding

Part:BBa_K211002:Experience

Designed by: ZHONG Wanting   Group: iGEM09_HKUST   (2009-10-13)
Revision as of 16:37, 18 October 2009 by Chuchu (Talk | contribs) (→‎HKUST2009 lab experience of BBa_K211002)

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HKUST2009 lab experience of BBa_K211002

 HKUST team in 2009 has characterized the BBa_K211002 in the two experiments as shown below. This chimeric odorant sensing GPCR has been proven to localize to the budding yeast membrane and upon binding to diacetyl, triggers downstream signalling pathway.

1.Membrane localization
(1)protocal
(2)[[results]]

[[Results]]
Upon inducing by Galactose for 45mins to 90mins, it is clear to see the membrane localization of the chimeric receptor in budding yeast.
[[Image:GPF2.jpg]]

2.Odorant sensing
(1)protocal
(2)results

Results
After being induced with galactose, the chimeric receptor is induced with its ligand diacetyl. From 0 to 4 hours, the cells sense the ligand and trigger the downstream reactions, leading to a cell cycle arrest at G1 phase. As a result, the DNA content is shifting towards the G1 non-replicated DNA content.
[[Image:FACS.jpg]]

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