Composite

Part:BBa_K4759204

Designed by: Jianghua Chen   Group: iGEM23_Jiangnan-China   (2023-10-07)
Revision as of 18:01, 7 October 2023 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


T7-RBS4-SUMO-linker-OleP

we add a solubilization-promoting tag at the N-end of the P450 enzyme, and in the selected six solubilization-promoting tags (SUMO, GST, MBP, TF, TRX, Nus)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 295
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 295
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 295
    Illegal BglII site found at 184
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 295
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 295
    Illegal AgeI site found at 420
    Illegal AgeI site found at 518
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None