Composite
Part:BBa_K4604018:Design
Designed by: Hannah Swientek Group: iGEM23_Freiburg (2023-10-07)
piG_02b (tetR_riboK12_mazF_mTurq)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 710
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 883
Illegal AgeI site found at 1376 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2250
Design Notes
Site directed mutagenesis was done between the tet promoter and RBS to remove a EcoRI restriction site.
Source
Cloned with Gibson Assembly. See more about exact sources on the pages of the BioBricks it is made up off.