Coding

Part:BBa_K4759004:Design

Designed by: Mengrui Tao   Group: iGEM23_Jiangnan-China   (2023-10-02)
Revision as of 14:47, 7 October 2023 by JianghuaChen (Talk | contribs) (→‎Source)

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BM3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1339
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 385
    Illegal BsaI.rc site found at 1309
    Illegal SapI.rc site found at 865
    Illegal SapI.rc site found at 1465


Design Notes

Recombinant plasmids pRSFDuet-BM3-GFP-1-10-GFP-11-olep, pRSFDuet-BM3-olep, etc



Source

The P450 BM3 reducing domain fraction derived from Bacillus megaterium

References