DNA

Part:BBa_K4727107

Designed by: Uni-Padua-IT 2023   Group: iGEM23_Uni-Padua-IT   (2023-09-15)
Revision as of 14:08, 7 October 2023 by Tommasovar (Talk | contribs)


sgDNA for OmpA125 in K. pneumoniae

This guide DNA has been designed to work with dCas9 to target AMR related genes in Klebsiella pneumoniae. The guide binds 125bp downstream the TSS, on the RV strand of the gene.

OmpA is a predominant outer membrane protein in Enterobacteriaceae. Its expression can be influenced by various environmental factors, among them the one exercised by glucose is the most discussed. In Klebsiella pneumoniae, KpOmpA mediates adhesion to a wide range of immune effector cells, facilitating respiratory tract and urinary infections. It stabilizes Gram-negative bacteria by anchoring their outer membrane to the peptidoglycan layer.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None