Part:BBa_K4897000
BS DNA-50 (BS DNA 1.0) using in L-RCA for detecting P. acne
What is it?
BS DNA-50 was designed by BS United China as a single-stranded DNA segment complementary to the 131 base pairs of the 16S rRNA gene of P. acne. The composition of the DNA has three categories: binding region (two ends), amplification region, and random region. The binding region is the key element of binding the P. acne 16s rRNA gene. The DNA ligase will perform the ligation of the single-strand DNA meanwhile the phi29 will generate double-stranded DNA through amplification primers
Usage and Biology
BS DNA-322 is the red line like a padlock. The black sequence represents the P. acne’s DNA specific to the binding. The red boxes represent the binding regions of the two ends of BS DNA50 (notice that the two ends are not connected). The purple boxes represent the random sequences generated. The Blue box represents the amplification region for RCA.
Overall, theoretically, through ligation and rolling circle amplification (L-RCA), BS DNA-50 should have high selectivity for detecting the 16S rRNA gene of P. acne.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 27
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 27
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 27
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 27
- 1000COMPATIBLE WITH RFC[1000]
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