Part:BBa_K4897005
Worm L-RCA (BS DNA 4.0) for detecting Myotonic Dystrophy
What is it?
Worm L-RCA is a novel detection technique based on our previous development of parts detecting P. acne. Our collaborators were very informative and interested in our research in L-RCA. After they successfully replicated our results, they trusted our quality and creativity and asked us for novel detection or diagnosis of certain human diseases. One of the diseases is called Myotonic Dystrophy which is hard to diagnose in the current stage. Here is the general description. Myotonic Dystrophy has different types according to its severity which is based on the amount of repetitions of 5’ CTG 3’. Myotonic Dystrophy Type 1 (DM1) is more severe and has more repetitions than Myotonic Dystrophy Type 2 (DM2). Due to the variability of the disease gene, the current method is flawed in its accuracy and time duration. However, Worm L-RCA is able to solve this issue. It has two DNA fragments working: BS Worm DNA-300 and BS Worm DNA-12. BS Worm DNA-300 is designed and is similar to previous DNAs which bind to P. acne’s DNA. It has the following composition: binding region, amplification region, and random region.
Usage and Biology
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 218
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 218
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 218
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 218
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 255
Illegal BsaI.rc site found at 13
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