Coding

Part:BBa_K4645003

Designed by: Zekun Luo   Group: iGEM23_HZAU-China   (2023-10-07)
Revision as of 09:34, 7 October 2023 by Registry (Talk | contribs)

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Fusion protein formed by CsgA and Hsa with (GGGGS)4 as linker.

The NR2 domain of Hsa is the sialic-binding core of Hsa protein, and CsgA is the extracellular fiber component of Escherichia coli biofilm formation. The two proteins were connected with a flexible linker to enable them to colonize on cat teeth and form a stable biofilm, creating conditions for the realization of subsequent neutralization.A 6x His tag was added to the C-terminus of the protein for purification or specificity testing in experiments.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 37
    Illegal PstI site found at 861
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 37
    Illegal PstI site found at 861
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 37
    Illegal PstI site found at 861
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 37
    Illegal PstI site found at 861
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None