Primer

Part:BBa_K4926006

Designed by: Wang Leyi   Group: iGEM23_PINGHE   (2023-10-07)
Revision as of 09:15, 7 October 2023 by Registry (Talk | contribs)

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GbcAsg1

cas12a needs to bind to crRNA to be active, and the sequence of the crRNA complementary to its target DNA determines the recognition targeting of cas12a. This is the primer used for in vitro transcription to get the corresponding crRNA of the target sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None