Regulatory
Part:BBa_K190022
Designed by: Michael Verhoeven Group: iGEM09_Groningen (2009-08-25)
Revision as of 14:49, 18 October 2009 by PaulSchavemaker (Talk | contribs)
Zinc Promoter (ZntR regulated) with own RBS
The pZntR from E.coli K.12 has a specific RBS site behind it in the genome. Here the RBS site is attachted to the promoter region. The RBS site might influence the activity of the promoter and will be tested in the same way as BBa_K190016. ZntR activates transcription when Zn(II) is bound (1).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
1) C.E. Outten, F.W. Outten, T.V. O’Halloran (1999) DNA Distortion Mechanism for Transcriptional Activation by ZntR, a Zn(II)-responsive MerR Homologue in Escherichia coli, The Journal of Biological Chemistry, Vol. 274, No. 53, Issue of December 31, pp. 37517–37524.
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Categories
Parameters
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