Composite

Part:BBa_K4767017:Design

Designed by: Fangjun Liu   Group: iGEM23_CUG-China   (2023-10-04)
Revision as of 08:31, 7 October 2023 by Xiang li8 (Talk | contribs) (→‎Source)


Pbad-RBS-luxI-TT-Ptac-luxR-TT-PluxI-gfp-TT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1872
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3021
    Illegal BsaI.rc site found at 3728
    Illegal SapI site found at 961


Design Notes

consideration


Source

Assembled by Subparts:AraK808000, BBa_J34801, luxIC0061, M31370, BBa_C0062, BBa_R0062, BBa_E0040.

References

Nistala, G.J., et al., A modular positive feedback-based gene amplifier. J Biol Eng, 2010. 4: p. 4.