Composite
Part:BBa_K4767016
Designed by: Fangjun Liu Group: iGEM23_CUG-China (2023-10-04)
RBS-TT-Pars-RBS-arsR-luxR(△2-162)
Uses a factor Pars(BBa_K4767001) ,strong RBS(BBa_J34801), DNA binding transcriptional repressor arsR(BBa_J15101), luxR(△2-162)(BBa_K4767000) and TT(BBa_B015).
Usage and Biology
This part is a Intermediate products for the construction of the luxR(△2-162) amplifier.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 427
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Nistala, G.J., et al., A modular positive feedback-based gene amplifier. J Biol Eng, 2010. 4: p. 4.
Xiaoqiang Jia, Bu Rongrong, Zhao Tingting, et al. Sensitive and Specific Whole-Cell Biosensor for Arsenic Detection[J]. Applied and environmental microbiology, 2019, 85(11): 1.
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Categories
Parameters
//classic/reporter
biology | Escherichia coli & Vibrio fischeri |