Composite

Part:BBa_K4767012:Design

Designed by: Fangjun Liu   Group: iGEM23_CUG-China   (2023-10-04)
Revision as of 07:43, 7 October 2023 by Xiang li8 (Talk | contribs) (→‎Design Notes)


Pars-RBS-arsR-RBS-luxR(△2-162)-TT-PluxⅠ-RBS-luxR(△2-162)-RBS-mtrC -TT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 270
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1543
    Illegal BsaI.rc site found at 929


Design Notes

We fused the arsenic responding biosensor and the LuxR-based amplifier, using mtrC as a target gene to detect arsenic in the chassis of Shewanella oneidensis in a electroactive biofilm-based sensor (EAB-sensor)

Source

Assembled by BBa_K4767001, BBa_J34801 , BBa_J15101 , BBa_B015,BBa_R0062, BBa_K3102020 and BBa_K4767000.

References

Xiaoqiang Jia, Bu Rongrong, Zhao Tingting, et al. Sensitive and Specific Whole-Cell Biosensor for Arsenic Detection[J]. Applied and environmental microbiology, 2019, 85(11): 1.

Nistala, G.J., et al., A modular positive feedback-based gene amplifier. J Biol Eng, 2010. 4: p. 4.