Coding

Part:BBa_K4724014

Designed by: Qingqing Zheng   Group: iGEM23_NJTech-China-A   (2023-10-07)
Revision as of 07:11, 7 October 2023 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


IsPETaseW185F-6xHis Tag


We mutate tryptophan into phenylalanine. We chose this mutation because the volume of tryptophan is very large, mutation into a small volume of phenylalanine can reduce the steric hindrance of substrate binding to enzymes, and phenylalanine can form large ∏ bonds with PET.



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 56
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 56
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 790
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 56
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 56
    Illegal AgeI site found at 546
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None