Part:BBa_K4767010

PluxⅠ-RBS-luxR(△2-162)-RBS-mtrC-TT

Uses a promotor PluxⅠ(BBa_R0062) ,strong RBS(BBa_J34801), luxR(△2-162)(BBa_K4767000), mtrC (BBa_K3102020)and TT(BBa_B015). This composite part is a BioBrick.

Usage and Biology

This is the response part of the luxR amplifier with the mtrC gene as the reporter. In our design, if luxR(△2-162) was expressed as a result of responding, this part can be activated to express mtrC and luxR(△2-162). MtrC will increase the ability for the Shewanella oneidensis to transfer the electrons, and luxR(△2-162) will functions in a positive feedback loop as it can bind to the PluxI promoter and activate its own transcription.

File:BBa K4767010.jpg

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 612
  

References

Nistala, G.J., et al., A modular positive feedback-based gene amplifier. J Biol Eng, 2010. 4: p. 4.