Coding
Part:BBa_K4724013
Designed by: Qingqing Zheng Group: iGEM23_NJTech-China-A (2023-10-07)
IsPETaseW159H-6xHis Tag
We mutate tryptophan into histidine. We chose this mutation because the R group of tryptophan is large, mutating into a small volume of histidine can reduce the steric hindrance of substrate binding to enzymes, and histidine is positively charged and can attract PET surfaces.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 56
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 56
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 790
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 56
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 56
Illegal AgeI site found at 546 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |