Not Released
Experience: Works
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Device
GluColi

Part:BBa_K196014

Designed by: Laetitia Warny   Group: iGEM09_ULB-Brussels   (2009-10-18)
Revision as of 13:43, 18 October 2009 by Laetitia (Talk | contribs)

Final GluColi device.

This is the part we actually created to test the glue production by E. coli. HfsG and HfsH come from Caulobacter crescentus. Caulobacter crescentus is an aquatic, Gram-negative bacterium that divides asymmetrically and is able to synthetize a strong glue. This glue is mainly made of a polysaccharide. There are different proteins needed to synthetize, export and attach it to the stalk of C. crescentus. To see the hole system, please see our wiki page [1]. In our project, we would like this glue to be produced by Escherichia coli. As E. coli does have similar genes for many of these proteins, but not for HfsG and HfsH, we decided to create a plasmid including only the genes coding for these two proteins. HfsG is a glycosyltransferase and HfsH is a carbohydrate esterase. RFP has been added as a reporter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2263
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1273
    Illegal AgeI site found at 636
    Illegal AgeI site found at 748
    Illegal AgeI site found at 1172
    Illegal AgeI site found at 1657
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme
//function/biosynthesis
//plasmidbackbone/copynumber/high
Parameters
None