DNA

Part:BBa_K4897016

Designed by: ZHANG TONG   Group: iGEM23_BS-United-China   (2023-10-07)
Revision as of 06:22, 7 October 2023 by Registry (Talk | contribs)

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sgRNA-R

This is a unique sgRNA contained by the Cas9 protein artificially added in our engineering E. coli, to ensure the blue light suicide system could successfully identify the target DNA region for gene editing and eventually kill the E. coli. The sgRNA-R in CAS9 is designed by similar methods as BBa_K4897015, except for the fact that it is used to target DNA-directed RNA polymerase subunit beta which serves as the building block of RNA polymerase in E. coli. Without the enzyme catalysing the transcription of DNA into RNA, E. coli would not be able to produce the proteins needed for its survival and would thus die. The specific sgRNA-R sequence is CGAGAAAAAACGTATTCGTAAGG. The CAS9 protein targeting the parts involved in essential bacterial metabolism serve as a strong double-layered protection to the environment, as the engineering E. coli will be deactivated as soon as CAS9 is activated.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None