Composite

Part:BBa_K4634006

Designed by: Ng Swee Hong   Group: iGEM23_Tsinghua   (2023-10-03)
Revision as of 05:18, 7 October 2023 by NgSweeHong (Talk | contribs)

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Histag OmpA mCherry

Histag GST OmpA mCherry is a fusion protein or construct in which a protein of interest is tagged with both a histidine tag (for purification purposes), a GST tag (for additional purification or binding applications), and is potentially directed to the bacterial outer membrane via the OmpA signal sequence. Additionally, the mCherry tag may allow for the visualization of the protein's location or expression within a cell or organism. This part is used for preliminary verification of secretion efficiency.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 751
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] Movva NR, Nakamura K, Inouye M. Amino acid sequence of the signal peptide of ompA protein, a major outer membrane protein of Escherichia coli. J Biol Chem. 1980 Jan 10;255(1):27-9. PMID: 6985608.

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