Part:BBa_K4897013
Dark suicide system based on Cas9
The blue light suicide system is a system that will start when our engineering bacteria lost in the external environment is not exposed to blue light. Since blue light is a type of visible light in the electromagnetic spectrum, its abundance in the natural environment is provided by common light sources such as sunlight, LED lights used in households, and fluorescent lights. The team thus investigated into how the myriad of blue light in the surroundings could be used to kill the used engineering E. coli for safety purposes.
By integrating this blue light suicide system into our engineering bacteria, precautions against potential leakage risks are established. Our engineering E. coli will not be able to survive in the external environment for more than a day, as when the night falls, it is forced to “commit suicide”. The detailed designs and procedures are illustrated in the figure below.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 5114
Illegal NheI site found at 5137
Illegal NheI site found at 5296
Illegal NheI site found at 5319
Illegal NheI site found at 5478
Illegal NheI site found at 5501 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 702
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 256
Illegal AgeI site found at 366 - 1000COMPATIBLE WITH RFC[1000]
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