Regulatory

Part:BBa_K239001:Design

Designed by: Axel Nystrom   Group: iGEM09_UCL_London   (2009-06-22)
Revision as of 13:19, 18 October 2009 by Axelnystrom (Talk | contribs) (→‎References)

Spy promoter, activated by Phosporylated CpxR, BaeR and Sigma70


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed. Sequence starts 3 bp before the first BaeR binding site and ends 5 pb after the -10 box.


Source

Genomic sequence from biocyc, Escherichia coli K-12. Native unmodified sequence.

References

A third envelope stress signal transduction pathway in Escherichia coli.

Robert G. Raffa and Tracy L. Raivio*. Molecular Microbiology (2002) 45(6), 1599–1611


Genome-Wide Analyses of Escherichia coli Gene Expression Responsive to the BaeSR Two-Component Regulatory System Kunihiko Nishino, Takeshi Honda, and Akihito Yamaguchi J Bacteriol. 2005 March; 187(5): 1763–1772. doi: 10.1128/JB.187.5.1763-1772.2005. PMCID: PMC1063996


The BaeSR two-component regulatory system mediates resistance to condensed tannins in Escherichia coli. Zoetendal EG, Smith AH, Sundset MA, Mackie RI. 2008 Appl Environ Microbiol. 2008 Jan;74(2):535-9. Epub 2007 Nov 26.


Global Analysis of Extracytoplasmic Stress Signaling in Escherichia coli Stéphanie Bury-Moné, Yanoura Nomane, Nancie Reymond, Romain Barbet, Eric Jacquet, Sandrine Imbeaud, Annick Jacq, and Philippe Bouloc PLoS Genet. 2009 September; 5(9): e1000651. Published online 2009 September 18. doi: 10.1371/journal.pgen.1000651. PMCID: PMC2731931


Signal Detection and Target Gene Induction by the CpxRA Two-Component System Patricia A. DiGiuseppe and Thomas J. Silhavy J Bacteriol. 2003 April; 185(8): 2432–2440. doi: 10.1128/JB.185.8.2432-2440.2003. PMCID: PMC152615

Nishino, K., T. Honda, and A. Yamaguchi. 2005. Genome-wide analyses of Escherichia coli gene expression responsive to the BaeSR two-component regulatory system. J. Bacteriol. 187:1763–1772.


Yamamoto K, Ogasawara H, Ishihama A (2008). "Involvement of multiple transcription factors for metal-induced spy gene expression in Escherichia coli." J Biotechnol 133(2):196-200. PMID: 17884222