![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4724010
Designed by: Qingqing Zheng Group: iGEM23_NJTech-China-A (2023-10-06)
IsPETaseQ126L-6xHis Tag
We turn glutamine into leucine. We chose for this mutation because it changed from hydrophilic amino acids to hydrophobic amino acids, promoting the formation of an active pocket hydrophobic environment.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 56
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 56
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 790
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 56
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 56
Illegal AgeI site found at 546 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |