Coding

Part:BBa_K4724008

Designed by: Qingqing Zheng   Group: iGEM23_NJTech-China-A   (2023-10-06)
Revision as of 23:37, 6 October 2023 by Registry (Talk | contribs)

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IsPETaseQ119F-6xHis Tag

We turn glutamine into phenylalanine. We chose this mutation because phenylalanine is a hydrophobic amino acid that promotes the formation of an active pocket hydrophobic environment. And phenylalanine has a benzene ring, which can form a large ∏ bond with PET.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 56
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 56
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 790
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 56
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 56
    Illegal AgeI site found at 546
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None