Composite

Part:BBa_K4634012:Design

Designed by: Ng Swee Hong   Group: iGEM23_Tsinghua   (2023-10-06)
Revision as of 17:55, 6 October 2023 by NgSweeHong (Talk | contribs) (→‎References)


OmpA-PD-L1 nanobody


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 316
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 316
    Illegal NotI site found at 269
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 316
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 316
    Illegal AgeI site found at 94
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NA


Source

OmpA: https://parts.igem.org/Part:BBa_K4380001 PD-L1 nanobody(BBa_K4634010): https://www.rcsb.org/structure/5DXW

References

[1] Gurbatri CR, Lia I, Vincent R, Coker C, Castro S, Treuting PM, Hinchliffe TE, Arpaia N, Danino T. Engineered probiotics for local tumor delivery of checkpoint blockade nanobodies. Sci Transl Med. 2020 Feb 12;12(530):eaax0876. doi: 10.1126/scitranslmed.aax0876. PMID: 32051224; PMCID: PMC7685004.
[2] Movva NR, Nakamura K, Inouye M. Amino acid sequence of the signal peptide of ompA protein, a major outer membrane protein of Escherichia coli. J Biol Chem. 1980 Jan 10;255(1):27-9. PMID: 6985608.