RNA
E1B4
Part:BBa_K273027
Designed by: Karl Brune Group: iGEM09_Uppsala-Sweden (2009-10-18)
Revision as of 11:52, 18 October 2009 by Karl.brune (Talk | contribs) (New page: __NOTOC__ <partinfo>BBa_K273027 short</partinfo> Antisense RNA against the formation of pyruvate dehydrogenase complex (PDC). Target is pyruvate dehydrogenase E1 (EC 1.2.4.1), in partucla...)
E1B4
Antisense RNA against the formation of pyruvate dehydrogenase complex (PDC). Target is pyruvate dehydrogenase E1
(EC 1.2.4.1), in partuclar the beta subunit (B). Antisense RNA approach #4.
Will be transcribed together with the BioBrick interface and may affect the performance of the antisense RNA molecule.
EXYZ
EX: E1 enzyme subunit of the PDC complex
Y: a=Alpha subunit of the particular enzyme subunit
b=beta subunit of the particular enzyme subunit
Z: # of antisense RNA approach
Usage and Biology
Antisense RNA function supposed to fuction through either Shine Dalgarno blocking and/or RNA elongation blocking.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 7
[edit]
Categories
Parameters
None |