Composite

Part:BBa_K4604015

Designed by: Hannah Swientek   Group: iGEM23_Freiburg   (2023-10-01)
Revision as of 16:32, 6 October 2023 by HannahSwientek (Talk | contribs)


piG_01b (tetR_bluB)

piG_01b is a plasmid consisting of the tet promoter/repressor, a functional bluB gene and the rrnB terminator. The backbone we used for experiments is pGGAselect. The tet promoter is a BioBrick from iGEM Freiburg 2022 (BBa_K4229059)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 710
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1618


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Categories
Parameters
None