Regulatory

Part:BBa_K233006:Design

Designed by: swetha srinivasan, chinar patil, mandar phatak, samit watve   Group: iGEM09_IBB_Pune   (2009-07-10)
Revision as of 11:44, 18 October 2009 by Samitwatve (Talk | contribs) (→‎Design Notes)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

AND Gate 3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We obtained a lac operator and paired it with phage T7 promoter. Hence we have an AND gate which requires both the inputs- T7 polymerase and IPTG/lactose in order for the gene downstream to be transcribed.

Source

E.coli phage t7

References