DNA
Part:BBa_K4800011:Design
Designed by: Yifei Wu Group: iGEM23_NJTech-China-B (2023-10-06)
YcjQ Donor
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 631
Illegal AgeI site found at 1313
Illegal AgeI site found at 1321 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1373
Design Notes
The genes at either end of the YcjQ gene were selected as the upper and lower homology arms and joined by overlap, the upper and lower homology arms were amplified separately, purified and recovered to obtain the YcjQ Donor.
Source
None.