DNA

Part:BBa_K4800011:Design

Designed by: Yifei Wu   Group: iGEM23_NJTech-China-B   (2023-10-06)
Revision as of 14:59, 6 October 2023 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


YcjQ Donor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 631
    Illegal AgeI site found at 1313
    Illegal AgeI site found at 1321
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1373


Design Notes

The genes at either end of the YcjQ gene were selected as the upper and lower homology arms and joined by overlap, the upper and lower homology arms were amplified separately, purified and recovered to obtain the YcjQ Donor.


Source

None.

References