Regulatory

Part:BBa_K233003:Design

Designed by: swetha srinivasan, samit watve, mandar phatak, chinar patil   Group: iGEM09_IBB_Pune   (2009-07-10)
Revision as of 11:35, 18 October 2009 by Samitwatve (Talk | contribs) (→‎Design Notes)

AND Gate 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

we had a lac binding site without any promoter activity synthesized. We obtained a lac operator and paired it with pLuxR. Hence we have an AND gate which requires both the inputs- LuxR/AHL and IPTG/lactose in order for the gene downstream to be transcribed

Source

Promoter biobricks Lac operator- E.coli

References