Coding
SIMD

Part:BBa_K4665005:Design

Designed by: Fien Eickmans   Group: iGEM23_MSP-Maastricht   (2023-10-01)
Revision as of 13:52, 6 October 2023 by FloorVervuren (Talk | contribs) (→‎References)


SazCA-INPN Membrane Display Module


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 470
    Illegal PstI site found at 592
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 470
    Illegal PstI site found at 592
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 470
    Illegal PstI site found at 592
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 470
    Illegal PstI site found at 592
    Illegal NgoMIV site found at 54
    Illegal AgeI site found at 555
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part results in a membrane fusion protein, meaning that extraction protocols for the protein should differ from normal extraction protocols


Source

SazCA is derived from Sulfurihydrogenibium azorense and INPN is derived from Pseudomonas syringae. Both sequences have been codon optimized for E. coli (BL21 DE3).

Our sequence for SazCa is derived from the paper by Zhu et al. (2022), which has been codon-optimized for E. coli BL21(DE3).

References

Zhu, Y., et al. (December 6, 2021). Surface display of carbonic anhydrase on Escherichia coli for CO2 capture and mineralisation. Synthetic and Systems biotechnology, 7(1): 460-473. https://doi.org/10.1016%2Fj.synbio.2021.11.008