Protein_Domain

Part:BBa_K203108

Designed by: Michael Bartoschek   Group: iGEM09_Heidelberg   (2009-10-15)
Revision as of 11:20, 18 October 2009 by MichaelBartoschek (Talk | contribs)

Plasma membrane targeting (Myrpalm)


Localization sequence to direct a protein into the cell membrane. This part has the BBb-standard restriction sites.

Myrpalm.jpg


Usage and Biology

This localization signal is located at the N-terminal end of the amino acid chain. The myrpalm signalsequence causes a myristoylation and palmitoylation of the targeted protein. Both modifications lead to a binding to the cell membrane.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 86
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 49
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 46


[edit]
Categories
Parameters
n/aPlasma membrane targeting (Myrpalm)