Part:BBa_K4677003
TorT-TorS
The TMAO reductase pathway is controlled by a receptor system that comprises the TMAO-binding protein TorT, the sensor histidine kinase TorS, and the response regulator TorR. TorT associates as a coreceptor with the histidine kinase receptor TorS to form the sensor complex. TorT-TorS is an asymmetric 2:2 complex that binds TMAO with negative cooperativity to form a symmetric active kinase. TMAO-stimulated TorS phosphorylates the response regulator TorR; phosphorylated TorR activates transcription from the torCAD operon to express TorCAD which are involved in the TMAO reductase pathway.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1361
Illegal XhoI site found at 3615 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 42
Illegal AgeI site found at 3366 - 1000COMPATIBLE WITH RFC[1000]
In order to construct the standard part pSB1C3-TorT-TorS plasmid, TorT-TorS sequence was tested to see if there is EcoRI and PstI site. The testing result was showed in Fig.1.
Fig.1 The map of TorT-TorS sequence described by SnapGene Viewer, showing the restriction enzyme information (no EcoRI and PstI sites).
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