Coding
Part:BBa_K4800015
Designed by: Chenxi Ma Group: iGEM23_NJTech-China-B (2023-10-06)
GabT
The promoter is Ptrc, which can be expressed in Escherichia coli KA30. YciA-sfp-MmCAR is derived from BBa_K1655000. The carboxylic acid reductase MmCAR position 302 is mutated with acid-base, and glutamine is replaced with glutamic acid. Obtain YciA-sfp-MmCAR (Q302E), improve the efficiency of carboxylic acid reduction, and YahK is an aldehyde reductase. This fragment constructs the 5-hydroxyvaleric acid to 1,5-pentanediol pathway.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 328
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |