![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4800007
Designed by: Chenxi Ma Group: iGEM23_NJTech-China-B (2023-10-06)
YciA
The promoter is Ptrc, which can be expressed in E. coli KA30. YciA-sfp-MmCAR was derived from BBa_K1655000, in which the carboxylic acid reductase MmCAR was acid-base mutated at position 302 to replace glutamine with glutamic acid to obtain YciA-sfp-MmCAR (Q302E), which improves the efficiency of carboxylic acid reduction. YahK is an aldehyde reductase. This fragment constructed the 5-hydroxyvaleric acid to 1,5-pentanediol pathway.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |