DNA

Part:BBa_K4800012

Designed by: Wenbin Zhao   Group: iGEM23_NJTech-China-B   (2023-10-06)
Revision as of 08:11, 6 October 2023 by Registry (Talk | contribs)

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ackA-pta sgRNA

SgRNA, or small guide RNA, is an important component of the CRISPR knockdown system. The N20 sequence of the acka-pta SgRNA is derived from the acka and pta genes and can be localised to the acka-pta gene in the KA30 genome to assist the Cas9 protein in silencing the gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None