Plasmid_Backbone

Part:BBa_K4579033:Design

Designed by: Alexa Morton   Group: iGEM23_Austin-UTexas   (2023-10-05)
Revision as of 05:15, 6 October 2023 by Alexakmorton (Talk | contribs) (→‎References)


pBTK1028 assembly backbone


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2237
    Illegal XbaI site found at 1229
    Illegal XbaI site found at 2246
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2237
    Illegal NheI site found at 2071
    Illegal NotI site found at 182
    Illegal NotI site found at 2225
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2237
    Illegal BglII site found at 1070
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2237
    Illegal XbaI site found at 1229
    Illegal XbaI site found at 2246
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2237
    Illegal XbaI site found at 1229
    Illegal XbaI site found at 2246
    Illegal NgoMIV site found at 105
    Illegal NgoMIV site found at 406
    Illegal AgeI site found at 1661
    Illegal AgeI site found at 1985
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 346
    Illegal SapI site found at 556


Design Notes

N/A - This part was not designed by the UT iGEM team.

Source

This plasmid comes from the Bee Toolkit, as described in: Leonard, S. P., Perutka, J., Powell, J. E., Geng, P., Richhart, D. D., Byrom, M., ... & Barrick, J. E. (2018). Genetic engineering of bee gut microbiome bacteria with a toolkit for modular assembly of broad-host-range plasmids. ACS Synthetic Biology, 7(5), 1279-1290.

References

[1] Leonard, S. P., Perutka, J., Powell, J. E., Geng, P., Richhart, D. D., Byrom, M., ... & Barrick, J. E. (2018). Genetic engineering of bee gut microbiome bacteria with a toolkit for modular assembly of broad-host-range plasmids. ACS Synthetic Biology, 7(5), 1279-1290.

[2] Lee, M. E., DeLoache, W. C., Cervantes, B., & Dueber, J. E. (2015). A highly characterized yeast toolkit for modular, multipart assembly. ACS Synthetic Biology, 4(9), 975-986.