Part:BBa_K177027
bax coding sequence
Human Bax CDS which a set of synonymous mutation made due to its optimal expression in Escherichia coli (changed codon bias).
The Bcl-2–associated X protein, or Bax is a protein of the Bcl-2 gene family. It promotes apoptosis by competing with Bcl-2 proper.
Bcl-2 family members share at least one of the four characteristic domains of homology entitled the Bcl-2 homology (BH) domains (named BH1, BH2, BH3 and BH4), and can form hetero- or homodimers. Bcl-2 proteins act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities.
Bax is a pro-apoptotic protein containing BH1, BH2 and BH3 domains. In healthy mammalian cells, the majority of Bax are presented in the cytosol, although upon initiation of apoptotic signaling, Bax undergoes a conformation shift, and inserts into organelle membranes, primarily the outer mitochondrial membrane. It is though that Bax induce the opening of the mitochondrial voltage-dependent anion channel, VDAC. This results in the release of cytochrome c and other pro-apoptotic factors from the mitochondria, often referred to as mitochondrial outer membrane permeabilization (MOMP), leading to activation of caspases which are responsible for further apoptotic changes of the cell.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 452
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| n/a | bax coding sequence |