Composite

Part:BBa_K4883004

Designed by: Yuqi Fu   Group: iGEM23_Hangzhou-BioX   (2023-06-29)
Revision as of 02:41, 6 October 2023 by Yuqi Fu (Talk | contribs)


Ptef1-RIB1-PTV-ADE4-Tcyc1

This is a complete expression cassette, constructed by inserting RIB1-PTV-ADE4 (BBa_K4883003) between a strong promoter Ptef1 and a CYC1 terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3298
    Illegal BamHI site found at 836
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 856
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 176



Usage and Biology

[edit]
Categories
Parameters
None