Part:BBa_K4883000

ADE4, phosphoribosylpyrophosphate amidotransferase from Saccharomyces cerevisiae

ADE4 encodes for phosphoribosylpyrophosphate amidotransferase in S. cerevisiae. This enzyme catalyzes the first step of the purine nucleotide biosynthetic pathway, directly forming inosine monophosphate (IMP) (Nieto & Woods, 1983).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Usage and Biology

ADE4 helps to synthesize IMP. IMP is the precursor of GTP, and GTP is the precusor of vitamin B2 (Kowalski et al., 2008; Gudipati et al., 2014). Overexpression of ADE4 could lead to overproduction of vitamin B2 in S. cerevisiae.

Figure 1. Gel electrophoresis of colony PCR products for verification of correct transformation of plasmid pCEV-G4-ADE4-Km into E. coli DH5α

References

Gudipati, V.; Koch, K.; Lienhart, W.-D.; Macheroux, P. The Flavoproteome of the Yeast Saccharomyces Cerevisiae. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 2014, 1844 (3), 535–544. DOI:10.1016/j.bbapap.2013.12.015.

Kowalski, D.; Pendyala, L.; Daignan-Fornier, B.; Howell, S. B.; Huang, R.-Y. Dysregulation of Purine Nucleotide Biosynthesis Pathways Modulates Cisplatin Cytotoxicity in Saccharomyces Cerevisiae. Molecular Pharmacology 2008, 74 (4), 1092–1100. DOI:10.1124/mol.108.048256.

Nieto DJ, Woods RA. Studies on mutants affecting amidophosphoribosyltransferase activity in Saccharomyces cerevisiae. Can J Microbiol. 1983 Jun;29(6):681-8. doi: 10.1139/m83-111. PMID: 6349762.