Part:BBa_K4579033

pBTK1028 assembly backbone

A type 5-1 entry vector part that can be used to create assemblies by combining with type 2, 3, and 4 parts according to the Bee Toolkit (BTK) standard. This backbone was created for the BTK by Shaunak Kar, with permission for the UT iGEM team to use it as a basis for our composite part assemblies.

For additional info on the BTK standard, see: Leonard, S. P., Perutka, J., Powell, J. E., Geng, P., Richhart, D. D., Byrom, M., ... & Barrick, J. E. (2018). Genetic engineering of bee gut microbiome bacteria with a toolkit for modular assembly of broad-host-range plasmids. ACS synthetic biology, 7(5), 1279-1290.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2237
  Illegal XbaI site found at 1229
  Illegal XbaI site found at 2246
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2237
  Illegal NheI site found at 2071
  Illegal NotI site found at 182
  Illegal NotI site found at 2225
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2237
  Illegal BglII site found at 1070
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2237
  Illegal XbaI site found at 1229
  Illegal XbaI site found at 2246
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2237
  Illegal XbaI site found at 1229
  Illegal XbaI site found at 2246
  Illegal NgoMIV site found at 105
  Illegal NgoMIV site found at 406
  Illegal AgeI site found at 1661
  Illegal AgeI site found at 1985
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal SapI site found at 346
  Illegal SapI site found at 556